How can the answer be improved? alveolar epithelial cell differentiation Flow cytometry sorting of NKX21 GFP SFTPC tdTomato cells enriched for expression of both NKX21 and SFTPC transcripts (Figure S1D); however, both the efficiency of differentiation and SFTPC expression levels within the SFTPC tdTomato population, compared with primary fetal alveolar epithelial control cells, were initially low (Figures S1D and S2).
Extracellular matrixdriven alveolar epithelial cell differentiation in vitro. During homeostasis and in response to injury, alveolar type II (AT2) cells serve as progenitor cells to proliferate, migrate, differentiate, and reestablish both alveolar type I (AT1) and AT2 cells into a functional alveolar epithelium. alveolar epithelial cell differentiation
Lung alveolar epithelium in vivo is composed of two specialized epithelial cell types, the squamous alveolar epithelial type I (ATI) cell, which constitutes approxi mately 93 of the alveolar epithelial surface area, and the surfactantproducing cuboidal alveolar epithelial type II Differentiated Human Alveolar Epithelial Cells and Reversibility of their Phenotype In Vitro. Correspondence and requests for reprints should be addressed to Robert J. Mason, M. D. , National Jewish and Medical Research Center, 1400 Jackson Street, Denver, CO. However, the effect of ubiquitously overexpressing Fgf10 on branching morphogenesis and alveolar epithelial cell differentiation requires further investigation since these lungs show an abnormal branching pattern and impaired proximaldistal epithelial differentiation after E13. 5. alveolar epithelial cell differentiation Abstract. In the Foxc2 knockout lungs, the capillary beds remained distant from the alveolar epithelium until the late stages, the number of type2 alveolar cells per alveolar progenitor cell was lower and the type1 alveolar cells were thicker in Foxc2 knockout mice. In contrast, Foxc2 expression was only detected in the mesenchyme